Genome cryoconservation of Russian Far East plants by seeds, spores and pollen frozen in liquid nitrogen|
Svetlana V. NESTEROVA1, Irina B. KRESHCHENOK2 & Irina N. KRESTOVA1
1 Botanical Garden-Institute FEB RAS, Vladivostok, Russia
2 Amur Branch of Botanical Garden-Institute FEB RAS, Blagoveshchensk, Russia
Long-term storage gene banks are a promising ex situway to preserve the wealth
of natural flora diversity. We investigated the possibility of cryopreservation using
liquid nitrogen (-196°C) for seeds, spores and pollen. The seeds of the species
studied retained their viability after thawing. However, a differential response of
seeds to deep freezing was detected. Statistical analysis did not reveal changes
in seed germination of species from the genera Agastache, Artemisia, Deutzia, Hypericum,
Potentilla, Pinus, Papaver, Primula, Rabdosia, Thymus, Weigela.
Seed germination of Ampelopsis and Celastrus species increased by 15-20 %.
Saxifraga and Rhododendron seeds also responded positively. An increase in
seed germination in Astragalus and Lespedeza could be explained by a decline
in the proportion of hard seeds. Liquid nitrogen had a negative thermal effect
on seeds in the genera Aristolochia, Armeniaca, Cerasus, and Microcerasus as
indicated by a decrease in germination. Cryopreservation did not affect growth
rate and development of plants when plants were grown from the frozen seeds.
Although fern spores responded differently to freezing, in the majority of the
species spore germination capacity remained unchanged after thawing. However,
in some species we observed an increase in germination by 60-80 % and reduced
spore germination by more than 40 %. It was revealed that the species of
the same genus or family respond differently to spore freezing. Frozen pollen of
Hemerocallis wild species and cultivars were used in the selection experiment with
the cv. "Cork". When using thawed pollen of cv. "Regal Air" the number of ovules
and seeds in the fruit increased. In the case of pollination with pollen cv. "Prairie
Blue Eyes" seed germination also increased. Flowering plants were grown from
the seeds obtained in these pollination experiments.
© 2012 Organizing Committee