Botanica Pacifica. A journal of plant science and conservation 2026. Preprint
Article first published online: 09 SEP 2026 | DOI: 10.17581/bp.2026.15211
Comparative phytochemical profiling of Crinum latifolium leaf extracts obtained by supercritical CO2 extraction and ethanol maceration using HPLC-ESI-MS/MS
Viet Cuong Tran 1
,
Mayya P. Razgonova 1,2 &
Kirill S. Golokhvast 2,3,4 1 Far Eastern Federal University, Vladivostok, Russia
2 N.I. Vavilov All-Russian Institute of Plant Genetic Resources, St. Petersburg, Russia
3 Siberian Federal Scientific Centre of Agrobiotechnology RAS, Krasnoobsk, Russia
4 Tomsk State University, Tomsk, Russia
Crinum latifolium L. is an Amaryllidaceae medicinal plant known for its alkaloid constituents, but its detectable phytochemical profile may vary with extraction conditions. We compared leaf extracts obtained by supercritical CO2 (SC-CO2) extraction and ethanol maceration using HPLC-ESI-MS/MS. We tentatively annotated 98 compounds in 12 chemical classes, with flavonoids and flavonoid glycosides forming the largest class, followed by alkaloids and phenolic acids. We detected 68 compounds in the SC-CO2 extract and 42 in the ethanol extract, with 12 occurring in both, giving a Jaccard similarity index of 0.122. Based on the literature examined, 66 compounds represent potential first records for C. latifolium. The two extraction procedures produced distinct but complementary detectable profiles, and the broader chemical coverage observed for SC-CO2 under these conditions does not imply higher compound concentrations or extraction yields.
Чан В.К.*, Разгонова М.П., Голохваст К.С. Сравнительный фитохимический профиль экстрактов листьев Crinum latifolium, полученных методом сверхкритической CO2-экстракции и этанольной мацерации, с использованием ВЭЖХ-ЭСИ-МС/МС. Crinum latifolium L. – лекарственное растение семейства Amaryllidaceae, известное как источник алкалоидов; при этом его выявляемый фитохимический профиль может зависеть от условий экстракции. Мы сравнили экстракты листьев, полученные методом сверхкритической CO2-экстракции (SC-CO2) и мацерации этанолом, с использованием ВЭЖХ-ЭСИ-МС/МС. В общей сложности мы предварительно аннотировали 98 соединений, относящихся к 12 химическим классам; наиболее представленной группой были флавоноиды и флавоноидные гликозиды, за которыми следовали алкалоиды и фенольные кислоты. В SC-CO2-экстракте было обнаружено 68 соединений, а в этанольном экстракте 42, причем 12 соединений были общими для обоих экстрактов; индекс сходства Жаккара составил 0,122. На основании изученной литературы 66 соединений можно рассматривать как предполагаемые первые находки для C. latifolium. Два метода экстракции дали различающиеся, но взаимодополняющие фитохимические профили. Более широкий химический охват, наблюдаемый для SC-CO2-экстракта в использованных условиях, не свидетельствует о более высоких концентрациях отдельных соединений или большем выходе экстракта.
Keywords: Crinum latifolium, Amaryllidaceae alkaloids, supercritical CO2 extraction, ethanol maceration, comparative phytochemical profiling, HPLC-ESI-MS/MS, flavonoids, алкалоиды амариллисовых, экстракция сверхкритическим CO2, мацерация этанолом, сравнительное фитохимическое профилирование, ВЭЖХ-ЭСИ-МС/МС, флавоноиды
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