Botanica Pacifica

Research paper

Botanica Pacifica. A journal of plant science and conservation 2024. Preprint
Article first published online: 02 DEC 2023 | DOI: 10.17581/bp.2024.13105

The recovery of Selaginella flaccida based on morphological and molecular data, with the description of S. khokhrjakovii from Indo-China and Peninsular Malaysia

Aleksandr P. Shalimov 1 ORCID, Vladimir F. Zaikov 1 ORCID, Sergey S. Kalyuzhny 2 ORCID, Meng-Hua Zhang 3 ORCID & Xian-Chun Zhang 4 ORCID

1 South Siberian Botanical Garden, Altai State University, Barnaul, Russia
2 Nikitsky Botanical Gardens – National Scientific Center RAS, Republic of the Crimea, Yalta, Russia
3 Key Laboratory of Plant Resources Conservation and Utilization, College of Biology and Environmental Sciences, Jishou University, Jishou, Hunan, China
4 State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany CAS, Beijing, China


Selaginella delicatula is one of the most widespread and polymorphic species in Asia. Recent molecular-phylogenetic studies have been shown that this species is not monophyletic. Traditionally, two species described from India as Lycopodium flaccidum Bory and L. curvatum Dalz. have long been recognized as synonyms of S. delicatula (Desv. ex Poir.) Alston. However, results of this study based on morphological and micromorphological data, coupled with molecular-phylogenetic evidences is showed that both of these synonyms represent a distinct species and resurrected here under name S. flaccida (Bory) Spring and to be distinct from S. delicatula. Furthermore, examination of the morphology of specimens collected in Vietnam and Cambodia, combined with additional examination of digital images from various locations in Indo-China and Peninsular Malaysia, has led us to describe a new species (S. khokhrjakovii Shalimov sp. nov.), which is similar to S. flaccida (Bory) Spring from India in size, habitus, general morphology, and lax branch system, but differs in leaf morphology and micromorphological features of mega- and microspores, which is also confirmed by molecular evidences.

Шалимов А.П., Зайков В.Ф., Калюжный С.С., Чжан М.-Х., Чжан С.-Ч. Восстановление Selaginella flaccida на основе морфологических и молекулярных анных, с описанием S. khokhrjakovii из Индокитая и полуострова Малайзия Семейство Collemataceae в лихенофлоре Магаданской области (Россия). Selaginella delicatula – один из самых распространенных и полиморфных видов в Азии. Недавние молекулярно-филогенетические исследования показали, что этот вид не монофилетичен. Традиционно, два вида описанных из Индии: Lycopodium flaccidum Bory и L. curvatum Dalz. давно признаны синонимами S. delicatula (Desv. ex Poir.) Alston. Однако результаты, основанные на данных морфологии и микромолфологии, в сочетании с молекулярно-филогенетическими доказательствами показали, что оба этих синонима представлены самостоятельным видом и восстанавливаются здесь под названием S. flaccida (Bory) Spring и отличаются от S. delicatula. Более того, изучение морфологии образцов, собранных во Вьетнаме, и Камбоджа, в сочетании с дополнительно изученными цифровыми изображениями из различных мест Индокитая и полуострова Малайзия, привело нас к описанию нового вида (S. khokhrjakovii Shalimov sp. nov.), который схож с S. flaccida (Bory) Spring из Индии в размере, габитусе, общей морфологии, и рыхлых веточках, но отличающиеся морфологией листьев, признаками микро морфологии мега- и микроспор, что также подтверждается молекулярными данными.

Keywords: Selaginella delicatula, taxonomy, morphology, spore morphology, synonymy, Selaginella delicatula, таксономия, морфология, морфология спор, синонимика

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