Botanica Pacifica

Research paper

Botanica Pacifica. A journal of plant science and conservation 2023. 12(2):155-162
Article first published online: 10 OCT 2023 | DOI: 10.17581/bp.2023.12209

Genetic diversity of the Rhododendron subsection Ledum species in Sakhalin Island: data from chloroplast DNA markers variability

Diana R. Iunusova1 ORCID, Maria A. Polezhaeva1 ORCID & Elena A. Marchuk2 ORCID

1 Institute of Plant and Animal Ecology UB RAS, Yekaterinburg, Russia
2 Botanical Institute-Garden FEB RAS, Vladivostok, Russia

A high genetic variability was revealed in the populations of the Rhododendron subsection Ledum species complex in Sakhalin. In total, 94 individuals from natural populations of the four sympatric species of Labrador tea (R. tomentosum, R. subarcticum, R. tolmachevii and R. hypoleucum) were analyzed to evaluate the genetic variation in the territory of the Sakhalin Island and in two mainland populations. The haplotype diversity of five chloroplast intergenic spacers (trnH–psbA, petB–petD, 3’trnV (UAC)–ndhC, K2R–K707, atpB–rbcL) was quite high. There were 16 haplotypes (h1–h16) identified; h1, h2 and h4 are the most common, the rest were rare and no species-specific ones were found. Analysis of Molecular Variance (AMOVA) indicate that about 37 % of the genetic diversity is due to among population differences (FST = 0.37, p < 0.001). Molecular phylogenetic analysis based on chloroplast DNA data did not reveal a clear species structure, but revealed a trend towards the isolation of two genetic groups: including a group of samples from the southern and the middle parts of Sakhalin Island.

Юнусова Д.Р., Полежаева М.А., Марчук Е.А. Генетическое разнообразие видов багульника на острове Сахалин: данные изменчивости маркеров хлоропластной ДНК. Изучено генетическое разнообразие 94 образцов из природных популяций четырех симпатричных видов багульников (подсекция Ledum рода Rhododendron: R. tomentosum, R. subarcticum, R. tolmachevii и R. hypoleucum) на территории о-ва Сахалин и в двух материковых популяциях. Изменчивость видов по пяти маркерным фрагментам хлоропластной ДНК (trnH–psbA, petB–petD, 3’trnV(UAC)–ndhC, K2R–K707, atpB–rbcL) оказалась довольно высокой. Выявлено 16 гаплотипов (h1-h16): h1, h2 и h4 – самые распространенные, остальные редкие, видоспецифичных не обнаружено. Анализ молекулярной дисперсии (AMOVA) показал, что около 37 % генетического разнообразия приходится на межпопуляционные различия (FST = 0,37, p < 0.001). Анализ родства, проведенный по данным хлоропластной ДНК, не выявил четкой видовой структуры, однако выявил тенденцию к обособлению двух генетических групп: включающей группу выборок из южной и средней частей о-ва Сахалин.

Keywords: cpDNA haplotypes, Ericaceae, hybridization, Labrador tea, population structure, Russian Far East, species complex, гаплотипы хпДНК, Ericaceae, гибридизация, багульник, структура популяции, Дальний Восток России, видовой комплекс



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