Botanica Pacifica. A journal of plant science and conservation 2022. 11(2):175-181
Article first published online: 7 SEP 2022 | DOI: 10.17581/bp.2022.11213
Diversity of filamentous fungi associated with Sargassum miyabei Yendo
Natalya N. Kirichuk
,
Victoriya Ye. Chausova &
Michael V. Pivkin G.B. Elyakov Pacific Institute of Bioorganic Chemistry FEB RAS, Vladivostok, Russia
We present data on the diversity of culturable fungi associated with brown algae Sargassum miyabei (the Sea of Japan, Russia). By the plate method, 269 isolates were obtained using solid nutrient media and 31 species from 12 genera were identified based on phenotypic as well as molecular genetic traits (using ITS and beta-tubulin markers) in cases where identification based on morphological features alone was difficult. The structure of the species composition was analyzed and the list of species was given. The analysis showed that facultative marine fungi were the vast majority of the species diversity, namely Penicillium, Cladosporium, Alternaria, Monodictys, Sarocladium, Trichoderma, Fusarium, Arthrinium, Botrytis and Aspergillus species. Among obligate marine fungi, Paradendryphiella and Piricauda species were revealed by the method mentioned above. Also, taxonomic affiliation and phylogenetic position of some Penicillium representatives have been clarified.
Киричук Н.Н., Чаусова В.Е., Пивкин М.В. Разнообразие мицелиальных грибов, ассоциированных с Sargassum miyabei Yendo. Представлены результаты изучения видового разнообразия микромицетов, ассоциированных с бурыми водорослями Sargassum miyabei (Японское море, Россия). Чашечным методом, с использованием плотных питательных сред, было выделено 269 изолятов грибов. На основе фенотипических, а также молекулярно-генетических признаков (генов ITS и бета-тубулина) идентифицирован 31 вид грибов из 12 родов. Проведен анализ структуры изученных грибных комплексов, а также приведен список видов. Анализ видового разнообразия показал доминирование факультативных морских грибов, в частности, выделены представители родов enicillium, Cladosporium, Alternaria, Monodictys, Sarocladium, Trichoderma, Fusarium, Arthrinium, Botrytis and Aspergillus species. Among obligate marine fungi, Paradendryphiella и Aspergillus. Из группы облигатных морских грибов выше упомянутыми методами были выявлены представители родов Paradendryphiella и Piricauda. Также, на основе молекулярных данных уточнены таксономическая принадлежность и филогенетическое положение некоторых видов Penicillium.
Keywords: marine mycology, fungal diversity, marine fungi, brown algae, Sargassum, phylogenetic analysis, ITS, beta-tubulin gene, морская микология, разнообразие микромицетов, морские мицелиальные грибы, бурые водоросли, филогенетический анализ, бета-тубулин
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