Botanica Pacifica. A journal of plant science and conservation 2022. 11(2):89-93
Article first published online: 17 OCT 2022 | DOI: 10.17581/bp.2022.11216
Phytoextraction of Chromium VI by Raphanus sativus L. under exogenous application of citric acid
Mohammed Bouhadi 1,2
,
M’hammed Elkouali 2 ,
Mohammed Talbi 2 ,
Fatiha Amegrissi 2 &
Hassan Fougrach 1 1 Laboratory of Ecology and Environment, Faculty of Sciences Ben M'sik, Hassan II University of Casablanca, Casablanca, Morocco
2 Laboratory of Analytical and Molecular Chemistry, Faculty of Sciences Ben M'sik, Hassan II University of Casablanca, Casablanca, Morocco
The pollution of environment by toxic heavy metals has increased dramatically over the past decades due to various sources of contamination, mainly industrial and human activities. Soil is the main recipient of air and water contamination. In this study, we evaluated the ability of radish plants to accumulate chromium VI (Cr(VI)) (150 ppm) under the application of citric acid (CA) (15 mM). The results showed that Cr(VI) reduced shoot dry weight, root dry weight, hypocotyl dry weight, and total chlorophyll content. On the other hand, Cr(VI) increased H2O2, malondialdehyde, and antioxidant activities of the radish plant. However, the application of CA improved the growth and physiological parameters and antioxidant content (superoxide dismutase 5 % and 4 %, peroxidase content 24 % and 4.25 %, catalase 38 % and 28 %, and ascorbate peroxidase 4 % and 35 %) in root and shoot part respectively, and increases the accumulation of Cr(VI) in different parts of the radish plant.
Бухади М., Элькуали М., Талби М., Амегрисси Ф., Фуграх Х. Фитоэкстракция хрома VI с помощью Raphanus sativus L. при экзогенном применении лимонной кислоты. За последние десятилетия загрязнение окружающей среды токсичными тяжелыми металлами из различных источников, главным образом, промышленной и человеческой деятельности, резко возросло. Почва является основным поглотителем загрязнений из воздуха и воды. В данном исследовании мы оценили способность растений редиса накапливать хром VI (Cr(VI)) (150 ppm) под действием лимонной кислоты (ЛК) (15 mM). Результаты показали, что Cr(VI) снижал сухой вес побегов, сухой вес корней, сухой вес гипокотиля и общее содержание хлорофилла. С другой стороны, Cr(VI) увеличил H2O2, малондиальдегид и антиоксидантную активность растений редиса. Однако применение ЛК улучшало рост и физиологические параметры и содержание антиоксидантов (супероксиддисмутазы 5 % и 4 %, пероксидазы 24 % и 4,25 %, каталазы 38 % и 28 %, аскорбатпероксидазы) 4 % и 35 %) в корнеплодах и побегах соответственно, и увеличивало накопление Cr(VI) в различных частях растения редиса.
Keywords: heavy metals, chromium VI, citric acid, antioxidants activities, radish, тяжелые металлы, хром VI, лимонная кислота, антиоксидантная активность, редис
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