Botanica Pacifica

Plant collections

Botanica Pacifica. A journal of plant science and conservation 2022
Article first published online: 07 MAY 2022 | DOI: 10.17581/bp.2022.11113 | EDN: IPHJOC

Genotyping NS and NSK herbaria specimens of Hedysarum (Fabaceae) using DNA sequencing

Natalia S. Nuzhdina ORCID & Nataliya K. Kovtonyuk ORCID

Central Siberian Botanical Garden SB RAS, Novosibirsk, Russia

Over 2000 specimens of the genus Hedysarum L., belonging to 48 species and 4 subspecies, were digitised and placed into CSBG SB RAS Digital herbarium whilst making a digital inventory of the herbarium collections of the Central Siberian Botanical Garden (NS, NSK). Totally, 466 nucleotide sequences were determined for 136 voucher specimens using the Sanger sequencing method. DNA sequences were obtained for 25 Hedysarum taxa for the first time.

Нуждина Н.С., Ковтонюк Н.К. Генотипирование образцов рода Hedysarum (Fabaceae) методом секвенирования ДНК в гербариях NS и NSK. Более 2000 гербарных образцов, принадлежащих 48 видам и 4 подвидам рода Hedysarum, были оцифрованы и размещены в Цифровом гербарии ЦСБС СО РАН при инвентаризации гербарных коллекций Центрального сибирского ботанического сада (NS, NSK). Для 136 ваучерных образцов были определены 466 последовательностей ДНК методом прямого секвенирования по Сэнгеру. Для 25 таксонов рода Hedysarum нуклеотидные последовательности определены впервые.

Keywords: biodiversity, digital herbarium, GBIF, NS, NSK herbarium collections, taxonomy, vascular plants, ITS, ETS, trnL-F, rpl32-trnL, rbcL, psbA-trnH, биоразнообразие, цифровой гербарий, гербарные коллекции, таксономия, сосудистые растения, молекулярные маркеры

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