Botanica Pacifica. A journal of plant science and conservation 2021.10(1):29-35
Article first published online: 10 MAY 2021 | DOI: 10.17581/bp.2021.10109
Genetic diversity of the endangered endemic species Hedysarum sangilense Krasnoborov et Timokhina (Fabaceae)
Inessa Y. Selyutina, Elena S. Konichenko*, Evgeniy G. Zibzeev & Irina N. Kuban
Central Siberian Botanical Garden SB RAS, Novosibirsk, Russia
Hedysarum sangilense Krasnoborov et Timokhina (Fabaceae) is а rare subendemic species of the Fabaceae family. It has been found in a restricted area, only in isolated habitats in Tyva Republic and Northern Mongolia. Two PCR techniques, using RAPD and inter-simple sequence repeat (ISSR) markers, were used to perform a comparative analysis of genetic diversity in this species. When amplifying DNA with three RAPD primers, we produced 51 bands, of which 21 (40.6 %) were polymorphic. Amplification of genomic DNA using ISSR analysis yielded 96 fragments, of which 35 (36.6 %) were polymorphic. Nei,s gene diversity (H) was estimated to be 0.105 within populations (range 0.086–0.150) and 0.191 at the species level. Genetic differentiation among populations (GST) was 0.383. The results indicate that both of the marker systems RAPD and ISSR, individually or in combination, can be effectively used in the determination of the genetic relationship among and within populations of H. sangilense.
Селютина И.Ю., Кониченко Е.С., Зибзеев Е.Г., Кубан И.Н. Генетическая изменчивость эндемичного вида Hedysarum sangilense Krasnoborov et Timokhina (Fabaceae). Hedysarum sangilense Krasnoborov et Timokhina (Fabaceae) – редкий геми-эндемичный вид семейства Fabaceae. Этот вид встречается на не большой территории, только в изолированных местообитаниях в Туве и Северной Монголии. Для анализа генетического разнообразия у этого вида использовались два метода ПЦР с использованием маркеров RAPD и ISSR. При амплификации ДНК с тремя праймерами RAPD получен 51 фрагмент, из которых 21 (31,04 %) был полиморфны. Амплификация геномной ДНК с использованием шести ISSR праймеров дала 96 фрагментов, из которых 35 (36,6 %) были полиморфными. Генетичекое разнообразие Nei (H) составило 0,105 внутри популяций (диапазон 0,086–0,150) и 0,191 на видовом уровне. Генетическая дифференциация между популяциями (GST) составила 0,383. Результаты показали, что обе маркерные системы RAPD и ISSR, по отдельности или в сочетании, могут быть эффективно использованы для выявления генетической изменчивости в популяциях и определения генетической взаимосвязи между популяциями H. sangilense.
Keywords: endangered species, genetic diversity, Hedysarum sangilense, ISSR, plant conservation, rare species, RAPD, subendemic, генетическое разнообразие, охрана растений, редкие виды, эндемик
References
Alexander, J.A., A. Liston & S. J. Popovich 2004. Genetic diversity of the narrow endemic Astragalus oniciformis (Fabaceae). American Journal of Botany 91(12):2004-2012. CrossRef
Bacchetta, G., G. Fenu, E. Mattana, G. Zecca, F. Grassi, G. Casazza & L. Minuto 2011. Genetic variability of the narrow endemic Rhamnus persicifolia Moris (Rhamnaceae) and its implications for conservation. Biochemical Systematic and Ecology 39:477-484. CrossRef
Barrett, S.C.H. & J.R. Kohn. 1991. Genetic and evolutionary consequences of small population size in plants: implications for conservation. In: Genetics and conservation of rare plants (D.A Falk & K.E. Holsinger, eds.), pp. 3-30, Oxford University Press, Oxford, UK.
Baskauf, C.J. & S. Snapp 1998. Population genetics of the cedar-glade endemic Astragalus bibullatus (Fabaceae) using isozymes. Annals of the Missouri Botanical Garden 85(1):90-96. CrossRef
Bauert, M.R., M. Kalin, M. Baltisberger, & P.J. Edwards 1998. No genetic variation detected within isolated relict populations of Saxifraga cernua in the Alps use RAPD markers. Molecular Ecology 7:1519-1527. CrossRef
Booy, G., R.J.J. Hendriks, M.J.M. Smulders, J.M. Van Groenendael & B. Vosman 2000. Genetic diversity and the survival of populations. Plant Biology 2(4):379-395. CrossRef
Cires, E., C. Cuesta & J.A. Fernandez Prieto 2013. Genetic diversity and structure in fragmented populations of the endangered species Ranunculus cabrerensis (Ranunculaceae): implication for conservation. Biologia 68(1):30-40. CrossRef
Dauber, J., J.C. Biesmeijer, D. Gabriel, W.E. Kunin, E. Lamborn, B. Meyer, A. Nielsen, S.G. Potts, S.P.M. Roberts, V. Sober, J. Settele, I. Steffan-Dewenter, J.C. Stout, T. Teder, T. Tscheulin, D. Vivarelli & T. Petanidou 2010. Effects of patch size and density on flower visitation and seed set of wild plants: a pan-European approach. Journal of Ecology 98(1):188-196. CrossRef
Despres, L., S. Loriot & M. Gaudeul 2002. Geographic pattern of genetic variation in the European globeflower Trollius europaeus L. (Ranunculaceae) inferred from amplified fragment length polymorphism markers. Molecular Ecology 11:2337-2347. CrossRef
Doyle, J.J. & J.L. Doyle 1987. A rapid DNA isolation of fresh leaf tissue. Phytochemical Bulletin 19(1):11-15.
Dudash, M.R. & C.B. Fenster 2000. Inbreeding and outbreeding depression in fragmented populations. In: Genetics, demography and viability of fragmented populations (A.G. Young & G.M. Clarc, eds.), pp. 35-53, Cambridge University Press, Cambridge. CrossRef
Edwards, A.L. & R.R. Sharitz 2000. Population genetics of two rare perennials in isolated wetlands: Sagittaria isoetiformis and S. teres (Alismataceae). American Journal of Botany 87(8):1147-1158. CrossRef
Ellstrand, N.C. & D.R. Elam 1993. Population genetic consequences of small population size: implications for plant conservation. Annual Review of Ecology, Evolution, and Systematics 24: 217-242. CrossRef
Escaravage, N., J. Cambecedes, G. Largier & A. Pornon 2011. Conservation genetics of the rare Pyreneo-Contabrian endemic Aster pyrenaeus (Asteraceae). AoB Plants 2011:plr029. CrossRef
Esselman, E. J., L. Jianqiang, D.J. Crawford, J.L. Windus & A.D. Wolfe 1999. Clonal diversity in the rare Calamgrositis porteri subsp. isperata (Poaceae): comparative results for allozymes and random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR) markers. Molecular Ecology 8(3):443-451. CrossRef
Falk, D.A. & K.E. Holsinger 1991. Genetic and conservation of rare plants. Oxford University Press, Ney York, 361 pp.
Frankham, R., J.D. Ballou & D.A. Briscoe 2002. Introduction to conservation genetics. Cambridge. Cambridge University Press, 617 pp. CrossRef
Gitzendanner, M.A. & P.S. Soltis 2000. Patterns of genetic variation in rare and widespread congeners. American Journal of Botany 87(6):783-792. CrossRef
Gaudeul, M, I. Till-Bottraud, F. Barjon & S. Manel 2004. Genetic diversity and differentiation in Eryngium alpinum L. (Apiaceae): comparison of AFLP and microsatellite markers. Heredity 92:508-518. CrossRef
Gunin, P.D., E.A. Vostokova, N.I. Dorofeyuk, P.E. Tarasova & C.C. Black 1999. Vegetation dynamics of Mongolia. (Geobotany 26). Kluwer Academic Publishers. Dordrecht. 238 pp. CrossRef
Gugerli, F., K. Eichenberger & J.J. Schneller 1999. Promiscuity in populations of the cushion plant Saxifraga oppositifolia in the Swiss Alps as inferred from RAPDs. Molecular Ecology 8:453-461. CrossRef
Hamrick, J.L. & J.W. Godt 1989. Allozyme diversity in plant species. In: Plant population genetics, breeding, and genetic resources (A.H.D. Brown, M.T. Clegg, A.L. Kahler & B.S. Weir, eds), pp. 43-63, Sinauer, Sunderland, MA.
Hamrick, J.L., M.J.W. Godt & S.L. Sherman-Broyles 1992. Factors influencing levels of genetic diversity in woody plant species. New Forests 6:95-124. CrossRef
Hamrick, J.L. & M.J.W. Godt 1996. Effects of life history traits on genetic diversity in plant species. Philosophical Transactions of the Royal Society B: Biological Sciences 351: 1291-1298. CrossRef
Heinrich, B. & G.A. Bartholomew 1978. Roles of endothermy and size in inter- and intraspecific competition for elephant dung in an African dung beetle, Scarabaeus laevistriatus. Physiological Zoology 52:484-494. CrossRef
Holsinger, K.E. & P. Vitt 1997. The future of conservation biology: what's a geneticist to do? In: Enhancing the ecological basis of conservation: heterogeneity, ecosystem function, and biodiversity (S.T.A. Pickett, R.S. Ostefeld, M. Shachak & G.E. Likens, eds), pp. 206-216, Chapman & Hall, New York. CrossRef
Hien,V.T.T & D.T. Phong 2012. Genetic diversity among endangered rare Dalbergia cochinchinensis (Fabaceae) genotypes in Vietnam revealed by random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers. African Journal of Biotechnology 11(35):8632-8644. CrossRef
Hughes, A.R., B.D. Inouye, M.T.J. Johnson, N. Underwood & M. Vellend 2008. Ecological consequences of genetic diversity. Ecology Letters 11(6):609-623. CrossRef
Ismail, S.A., J. Ghazoul, G. Ravikanth, R. Uma Shaanker, C.G. Kushalappa & C.J. Kettle 2012. Does long-distance pollen dispersal preclude inbreeding in tropical trees? Fragmentation genetics of Dysoxylum malabaricum in an agro-forest landscape. Molecular Ecology 21:5484-5496. CrossRef
Josiah C.C., D.O. George, O.M Eleazar & W.F. Nyamu 2008. Genetic diversity in Kenyan populations of Acacia senegal (L.) willd revealed by combined RAPD and ISSR markers. African Journal of Biotechnology 7(14):2333-2340.
Kalisz, S., F.M. Hanzawa, S.J. Tonsor, D.A. Thiede & S. Voigt 1999. Ant-mediated seed dispersal alters pattern of relatedness in a population of Trillium grandiflorum. Ecology 80: 2620-2634. CrossRef
Krasnikov, A. & D. Schaulo 2004. Karyoiogical study of the Tuva Republic flora: summary. Turczaninowia 7(2):82-95 (in Russian). [Красников А., Д. Шауло 2004. Кариологическое изучение флоры республики Тува: некоторые итоги // Turczaninowia. T.7, №2. C. 85-92].
Krasnoborov, I.M &. S.A. Timochina 1975. A new species of Hedysarum L. from the Tuva ASSR. Novitates Systematicae Plantarum Vascularum 12:234-237 (in Russian). [Красноборов И.М., Тимохина С.А 1975. Новый вид Hedysarum L. из Тувинской АССР // Новости систематики высших растений. №12. С. 234-237].
Kunin, W.E. 1997. Population size and density effects in pollination: pollinator foraging and plant reproductive success in experimental arrays of Brassica kaber. Journal of Ecology 85:225-234. CrossRef
Kurbatskii, V.I. 1994. Hedysarum L. In: Flora of Siberia, vol. 9 (A.V. Polozhii & L.I. Malyshev, eds), pp. 153-166, Nauka, Novosibirsk (in Russian). [Курбатский В.И. 1994. Hedysarum L. - Копеечник // Флора Сибири / под ред. А.В. Положий и Л.И. Малышева. Новосибирск: Наука. Т. 9. С. 153-166].
Kuss P, A.R. Pluess, H.H. Egisdottir & J. Stöcklin 2008. Spatial isolation and genetic differentiation in naturally fragmented plant populations of the Swiss Alps. Journal of Plant Ecology 1:149-159. CrossRef
Lazaro, A., R. Lundgren & O. Totland 2009. Co-flowering neighbors influence the diversity and identity of pollinator groups visiting plant species. Oikos 118(5):691-702. CrossRef
Levin, D.A. 1987. Local differentiation and the breeding structure of plant populations. In: Plant evolutionary biology (L.D. Gottlieb & S.K. Jain, eds), pp. 305-329, Chapman & Hall: London. Lewinton, R.C. 1972. The apportionment of human diversity. Evolutionary Biology 6:381-398. CrossRef
Loveless, M.D. & J.L. Hamrick 1984. Ecological determinations of genetic structure in plant populations. Annual Review of Ecology, Evolution, and Systematics 15:65-95. CrossRef
Malyshev, L.I. & K.A. Sobolevskaya (eds) 1980. Rare and endangered plants of Siberia, Nauka, Novosibirsk, 223 рp. (in Russian). [Редкие и исчезающие растения Сибири / под ред. Л.И. Малышева и К.А. Соболевской. Новосибирск: Наука. 223 с.].
Matties, D., I. Brauer, W. Maibom & T. Tscharntke 2004. Population size and the risk of local extinction: empirical evidence from rare plants. Oikos 105:481-488. CrossRef
McGlaughlin, M., K. Karoly & T. Kaye 2002. Genetic variation and its relationship to population size in reintroduced populations of pink sand verbena, Abronia umbellate subsp. breviflora (Nyctaginaceae). Conservation Genetics 3(4): 411-420. CrossRef
Milligan, B.G., J. Leebens-Mack & A.E. Strand 1994. Conservation genetics: beyond the maintenance of marker diversity. Molecular Ecology 3(4):423-435. CrossRef
Neel, M.C. 2008. Patch connectivity and genetic diversity conservation in the federally endangered and narrowly endemic plant species Astragalus albens (Fabaceae). Biological Conservation 141(4): 938-955. CrossRef
Nei, M. 1972. Analysis of gene diversity in subdivided populations. Proceeding of the National Academy of Science of the USA 70(12):3321-3323. CrossRef
Nybom, H. & I.V. Bartish 2000. Effects of life history traits and sampling strategies on genetic diversity estimates obtained with RAPD markers in plant. Perspective in Plant Ecology, Evolution and Systematics 3(2):93-114. CrossRef
Nybom, H. 2004. Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants. Molecular Ecology 13:1143-1155. CrossRef
Oostermeijer, J.G.B., S.H. Luijten, & J.C.M. den Nijs 2003. Integration demographic and genetic approaches in plant conservation. Biological Conservation 113:389-398. CrossRef
Oliva-Tejera, F., J. Caujapé-Castells, J. Naranjo-Suárez, J. Navarro-Déniz, J.R. Acebes-Ginovés & D. Bramwell 2005. Population genetic differentiation in taxa of Lotus (Fabaceae: Loteae) endemic to the Gran Canarian pine forest. Heredity 94(2):199-206. CrossRef
Peakall, R & P. Smouse 2012. GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research - an update. Bioinformatics 28:2537-2539. CrossRef
Peredo, E.L., M.A. Revila, B. Jimenez-Alfaro, A. Bueno, J.A.F. Prieto & R.J. Abbott 2009. Historical biogeography of a disjunctly distributed, Spanish alpine plant, Senecio boissieri (Asteraceae). Taxon 58:1-10. CrossRef
Pluess, A.R., & J. Stocklin 2004. Population genetic diversity of the clonal plant Geum reptans (Rosaceae) in the Swiss Alps. American Journal of Botany 91:2013-2021. CrossRef
Rathcke, B.J. & E.S. Jules 1993. Habitat fragmentation and plant-pollinator interactions. Current Science 65:273-277.
Rogenski, C. & M. Smith, E. Esselman 2009. ISSR marker diversity of the threatened Astragalus crassicarpus var. trichocalyx in Illinois populations. Transactions of the Illinois State Academy of Science 102 (3/4):149-159.
Schaal B.A, D.A. Hayworth, K.M. Olsen, J.T. Rauscher & W.A. Smith 1998. Phylogeographic studies in plants: problems and prospects. Molecular Ecology 7:465-474. CrossRef
Selyutina, I.Yu., E.S. Konichenko & O.V. Dorogina 2014. The possibility of using of ISSR-markers for detection of genetic differentiation of populations of rare species Astragalus sericeocanus (Fabaceae). Rastitel'nyi Mir Aziatskoi Rossii 4:3-8 (in Russian). [Селютина И.Ю., Кониченко Е.С., Дорогина О.В. 2014. Возможности использования ISSR-маркеров для выявления генетической дифференциации популяций редкого вида Astragalus sericeocanus (Fabaceae) 2014 // Растительный мир Азиатской России №4(16). С. 3-8.]
Selyutina, I.Yu., E.S. Konichenko, O.V. Dorogina & D.V. Sandanov 2016. Genetic diversity of the endangered endemic milkvetch Astragalus sericeocanus Gontsch., Fabaceae, from the Lake Baikal region. Biochemical Systematics and Ecology 63: 163-169. CrossRef
Schonswetter, P., A. Tribsch, M. Barfuss & H. Niklfeld 2002. Several Pleistocene refugia detected in the high alpine plant Phyteuma globulariifolium in the Europian Alps. Molecular Ecology 11:2637-2647. CrossRef
Schonswetter, P., A. Tribsch & H. Niklfeld 2004. Amplified Fragment Length Polymorphism (AFLP) reveals no genetic divergence of the Eastern Alpine endemic Oxytropis campestris subs. tiroliensis (Fabaceae) from widespread subs. campestris. Plant Systematics and Evolution 244(3):245-255. CrossRef
Slatkin, M. 1985. Gene flow in natural populations. Annual Review of Ecology, Evolution, and Systematics 16:393-430. CrossRef
Slatkin, M. 1987. Gene flow and the geographic structure of natural populations. Science 236:787-792. CrossRef
Travis, S.E., J. Maschinski & P. Keim 1996. An analysis of genetic variation in Astragalus cremnophylax var. cremnophylax, a critically endangered plant, using AFLP markers. Molecular Ecology 5(6):735-745. CrossRef
Urgamal, M., B. Oyuntsetseg, D. Nyambayar & Ch. Dulamsuren. 2014. Conspectus of the vascular plants of Mongolia. Admon Printing, Ulaanbaatar, Mongolia, 282 pp.
Van Dyke, F. 2008. Conservation biology: foundations, concepts, applications. 2nd edition. Springer, Berlin, 614 pp.
Vicente, M.J., F. Segura, M. Aguado, D. Migliaro, J.J. Franco & J.J. Martínez-Sánchez 2011. Genetic diversity of Astragalus nitidiflorus, a critically endangered endemic of SE Spain, and implications for its conservation. Biochemical Systematics and Ecology 39:175-182. CrossRef
Wallace, L.E. 2002. Examining the effects of fragmentation on genetic variation in Platanthera leucophaea (Orchidaceae): inference from allozyme and random amplified polymorphic DNA markers. Plant Species Biology 17:37-49. CrossRef
Wesche, K., I. Hensen & R. Undrakh 2006. Genetic structure of Galitzkya macrocarpa and G. potaninii, two closely related endemics of Central mountain ranges. Annals of Botany 98:1025-1034. CrossRef
Wilcock, C. & R. Neiland 2002. Pollination failure in plants: why it happens and when it matters. Trends in Plant Science 7(6): 270-277. CrossRef
Yeh, F.C., R.C. Yang, T.B.J. Boyle Z.H. Ye & J.X. Mao 1997. Popgene: the user friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Center, University of Alberta, Edmonton.
Zhang, Z., S. Chen, S. Chen & Q. Gao 2007. Patterns of genetic variation in Swertia przewalskii, an endangered endemic species of the Qinghai-Tibet Plateau. Biochemical Genetics 45(1-2):33-50. CrossRef
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