Anna A. Erst & Andrey S. Erst. In vitro propagation of Eranthis stellata (Ranunculacea), endemic species with narrow distribution in the Russian Far East, Northeast China and North Korea

Research papers

Botanica Pacifica. A journal of plant science and conservation 2020.9(2):121-125
Article first published online: 25 AUG 2020 | DOI: 10.17581/bp.2020.09210

In vitro propagation of Eranthis stellata (Ranunculacea), endemic species with narrow distribution in the Russian Far East, Northeast China and North Korea

Anna A. Erst¹ & Andrey S. Erst^1,2

¹ Central Siberian Botanical Garden SB RAS, Novosibirsk, Russia
² Tomsk State University, Tomsk, Russia

The rare species Eranthis stellata (Ranunculaceae) was cultured for the first time, for which the use of peduncles with underdeveloped flower buds as primary explants was found to be effective. Upon introduction to culture in vitro, explants should be cultured in the dark at low temperature (17°C) to prevent phenolic oxidation. A culture medium containing ½ MS supplemented with 5 μM 6-benzylaminopurine is optimal for the propagation of this species. A morphogenic response rate of 32 % was achieved, and the propagation rate amounted to 4.2 shoots per explant.

Эрст А.А., Эрст А.С. Размножение в культуре in vitro Eranthis stellata (Ranunculaceae), эндемичного вида с ограниченным распространением на Дальнем Востоке России, Северо-Востоке Китая и в Северной Корее. Впервые введен в культуру in vitro редкий вид Eranthis stellata, сем. Ranunculaceae. Показана эффективность использования в качестве первичных эксплантов цветоносов с недоразвитыми бутонами. Отмечено, что на этапе введения в культуру in vitro экспланты необходимо культивировать при пониженной температуре (+17°C) в темноте для преодоления фенольного окисления. Питательная среда ½ MS, дополненная 5 μM 6-бензиламинопукина, является оптимальной для размножения данного вида: морфогенный ответ составил 32 %, коэффициент размножения 4.2 шт./эксплант.

Keywords: in vitro culture, rare taxa, Eranthis stellata, micropropagation, conservation, редкий вид, микроразмножение, сохранение

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References

Akhare, A.A., D.B. Dhumale, S.B. Sakhare & S. Ekta 2008. Remove from marked records in vitro establishment of Gladiolus cv. White Friendship and Fidelio using axillary buds as explant. Asian Journal of Horticulture 3(1):149-152.

Babkina, S.V. & E.V. Safonova 2008. Eranthis stellata. In: Red Book of the Khabarovsk Territory (E.M. Nikitina & M.V. Salnikova, eds), pp. 223-224, Izdatel'skii dom Priamurskie vedomosti, Khabarovsk (in Russian). [Бабкина С.В., Сафонова Е.В. 2008. Eranthis stellata // Красная книга Хабаровского края / под ред. Е.М. Никитина, М.В. Сальникова. Хабаровск: Издательский дом Приамурские ведомости. С. 223-224].

Baskin, J.M. & C.C. Baskin 1986. Germination ecophysiology of the mesic deciduous forest herb Isopyrum biternatum. Botanical Gazette 147:152-155. CrossRef

Buchheim, J.A.T. & M.M. Meyer 1992. Micropropagation of peony (Paeonia spp.). In: Biotechnology in Agriculture and Forestry, vol. 20 (Y.P.S. Bajaj, ed.), pp. 269-285, Springer Verlag, Berlin, Heidelberg.

Bullowa, S. & Y. Ozeri 1975. Role of temperature and growth regulators in germination in Anemone coronaria L. Australian Journal of Plant Physiology 2:91-100. CrossRef

Cıg, A. & G. Basdogan 2015. In vitro propagation techniques for some geophyte ornamental plants with high economic value. International Journal of Secondary Metabolite 2(1):27-49.

Darman, G.F. 2009. Shibateranthis stellata. In: Red Book of the Amur Region: Rare and endangered species of animals, plants and fungi (O.N. Kozhemyako et al., eds), pp. 315-316, Izdatel'stvo BGPU, Blagoveshchensk (in Russian). [Дарман Г.Ф. 2009. Shibateranthis stellata // Красная книга Амурской области: Редкие и находящиеся под угрозой исчезновения виды животных, растений и грибов / под ред. Кожемяко О.Н. и др. Благовещенск: Издательство БГПУ. С. 315-316].

Dhooghe, E. & M.Ch. Van Labeke 2007. In vitro propagation of Helleborus species. Plant Cell, Tissue and Organ Culture 91: 175-177. CrossRef

Erst, A.A. & A.S. Erst 2019. Features of in vitro seed germination of Eranthis longistipitata, an endemic plant of Central Asia. Plant Cell Biotechnology and Molecular Biology 20:611-616.

Erst, A.S., A.P. Sukhorukov, E.Y. Mitrenina, M.V. Skaptsov, V.A. Kostikova, O.A. Chernisheva, V. Troshkina, M. Kushunina, D.A. Krivenko, H. Ikeda, K. Xiang & W. Wang 2020. An integrative taxonomic approach reveals a new species of Eranthis (Ranunculaceae) in North Asia. PhytoKeys 4(140):75-100. CrossRef

Forbis, T.A., S.K. Floyd & A.D. Queiroz 2002. The evolution of embryo size in angiosperms and other seed plants: implications for the evolution of seed dormancy. Evolution 56:2112-2125. CrossRef

Gamborg, O.L. & D.E. Eveleigh 1968. Culture methods and detection of glucanases in cultures of wheat and barley. Canadian Journal of Biochemistry 46(5):417-421. CrossRef

George, M.W. & R.R. Tripepi 2004. Micropropagation of Lewisia cotyledon using axillary buds from flower peduncles. Native Plants Journal 5(1):75-80. CrossRef

Hussey, G. 1977. In vitro propagation of Gladiolus by precocious axillary shoot formation. Scientia Horticulturae 6(4):287-296. CrossRef

Kulkhanova, D.S., A.A. Erst & T.I. Novikova 2015. In vitro regeneration from bulbous scales of Fritillaria sonnikovae, an endemic species. Russian Journal of Developmental Biology 46:215-221. CrossRef

Li, Y., D.Wu, S. Pan, S. Xu, Z. Wei, Z. Xu & X. Li 1984. In vitro propagation of Paeonia suffruticosa. Kexue Tongbao 29: 1675-1678.

Malyshev, L.I. 2005. Ranunculaceae. In: Conspectus florae Sibiriae: plantes vasculares (K.S. Baykov, ed.), pp. 20-35, Nauka, Novosibirsk (in Russian). [Малышев Л.И. 2005. Ranunculaceae // Конспект флоры Сибири: Сосудистые растения / под ред. К.С. Байкова. Новосибирск: Наука. С. 20-35].

Marcinkowski, J. 2002. Byliny ogrodowe - produkcja i zastosowanie. Państwowe Wydawnictwo Rolnicze i Leśne, Warszawa. 388 ss.

Mayorov, S.R. & Yu.K. Vinogradova 2013. Naturalization of plants in the botanical gardens of Moscow. Vestnik Udmurtskogo Universiteta 2:12-16 (in Russian). [Майоров С.Р., Виноградова Ю.К. 2013. Натурализация растений в ботанических садах г. Москвы // Вестник Удмуртского Университета. Вып. 2. C. 12-16].

Muraseva, D.S., T.I. Novikova & A.A. Erst 2015. In vitro propagation and conservation of rare species Fritillaria meleagris L. from floral explants. Contemporary Problems of Ecology 8:754-763. CrossRef

Muraseva, D.S. & T.I. Novikova. 2018. Efficient protocol for in vitro propagation from bulb scale explants of Fritillaria ruthenica Wikstr. (Liliaceae), a rare ornamental species. Rendiconti Lincei. Scienze Fisiche e Naturali 29:491-497. CrossRef

Murashige, T. & F. Skoog 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15:473-497. CrossRef

Nasırcılar, A.G., S. Mirici, O. Eren, O. Karagüzel & I. Baktır 2011. Micropropagation of Turkish endemic Iris stenophylla Hausskn & Siehe ex Baker subsp. allisonii B. Mathew. Acta Horticulturae 886:187-192. CrossRef

Nasircilar, A.G., S. Mirici, O. Karagüzel, O. Eren & I. Baktir 2011. In vitro propagation of endemic and endangered Muscari mirum from different explant types. Turkish Journal of Botany 35:37-43.

Nesterova, S.V. 2004. Cryopreservation of seeds of wild plants of the Primorsky Territory: abstract ... candidate of biological sciences, Vladivostok. 24 pp. (in Russian). [Нестерова С.В. 2004. Криоконсервация семян дикорастущих растений Приморского края: автореф...к.б.н., Владивосток, 24 с.]

Ozcan, S., I. Parmaksız, S. Mirici, S. Cocu, S. Uranbey, A. Ipek, C. Sancak, E. O. Sarıhan, B. Gurbuz, C., S. Sevimay, & N. Arslan 2007. Efficient in vitro bulblet regeneration from immature embryos of endemic and endangered geophyte species in Sternbergia, Muscari and Fritillaria genera. In: Biotechnology and sustainable agriculture 2006 and beyond (Z. Xu, J. Li, Y. Xue & W. Yang, eds), pp. 381-383, Springer, Netherlands, Dordrecht. CrossRef

Podwyszynska, M. 2012. The mechanisms of in vitro storage organ formation in ornamental geophytes. In: Floriculture and ornamental biotechnology. Bulbous ornamentals (J.M. Van Tuyl & P. Arens, eds), 6(1):9-23, Global Science Books, London, UK.

Seabrook, J.E.A. & B.G. Cumming 1977. The in vitro propagation of amaryllis (Hippeastrum spp. hybrids). In Vitro 13: 831-836. CrossRef

Semenova, G.P. 2007. Rare and endangered species of Siberian flora: biology, conservation. Geo, Novosibirsk, 408 pp. (in Russian). [Семенова Г.П. 2007. Редкие и исчезающие виды флоры Сибири: биология, охрана. Новосибирск: Гео. 408 с.].

Seyring, M. 2002. In vitro cloning of Helleborus niger. Plant Cell Reports 20:895-900. CrossRef

Sharanappaand, P. & V. Ravishankar Rai 2011. Micropropagation of Thalictrum dalzellii Hook. through rhizome buds. Journal of Phytology 3(5):51-55.

Shen, M., Q. Wang, X. N. Yu & J.A.T. da Silva 2012. Micropropagation of herbaceous peony (Paeonia lactiflora Pall.). Scientia Horticulturae 148:30-38. CrossRef

Sobolevskaya, K.A. 1984. Disappearing plants of Siberia in the introduction. Nauka, Novosibirsk, 220 pp. (in Russian). [Соболевская К.А. 1984. Исчезающие растения Сибири в интродукции. Новосибирск: Наука. 220 с.].

Tipirdamaz, R. & A. Gomurgen 2000. The effects of temperature and gibberellic acid on germination of Eranthis hyemalis (L.) Salisb. seeds. Turkish Journal of Botany 24: 143-145.

Wang, H. & J. van Staden 2001. Establishment of in vitro cultures of tree peonies. South African Journal of Botany 67:358-361. CrossRef

Wang, Q., H. Tang, Y. Quan & Y.Q. Tang 1994. Phenol induced browning and establishment of shoot-tip expiants of 'Fuji' apple and 'Jinhua' pear cultured in vitro. Journal of Horticultural Science 69(5):833-839. CrossRef

Yakubov, V.V. 2006. Eranthis stellata. In: Red Book of the Jewish Autonomous Region. Rare and endangered plant and fungi species (T.A. Rubtsova & S.D. Schlotgauer, eds), р. 124, DVO RAN, Inctitut kompleksnogo analiza regional'nykh problem, ARGA, Novosibirsk (in Russian). [Якубов В.В. 2006. Eranthis stellata // Красная книга Еврейской автономной области. Редкие и и находящиеся под угрозой исчезновения виды растений и грибов / под ред. Т.А. Рубцовой, С.Д. Шлотгауэра. ДВО РАН, Ин-т комплекс. анализа регион. проблем, Новосибирск: АРГА. С. 248.

Zhao, L.L., H. Ge, C.H. Fan, F. Yin, Q.X. Li & Y.Y. Zhou 2006. Effects of pH and temperature on browning of Phalaenopsis explants cultured in vitro. Acta Horticulturae Sinica 33(6):1373-1376.

Ziv, M. & H. Lilien-Kipnis 2000. Bud regeneration from inflorescence explants for rapid propagation of geophytes in vitro. Plant Cell Reports 19:845-850. CrossRef