Botanica Pacifica. A journal of plant science and conservation Preprint
Article first published online: 31 MAY 2019 | DOI: 10.17581/bp.2019.08202
Phenolic compounds and antimicrobial properties of Begonia grandis Dryand. subsp. grandis leaves
Evgeniya A. Karpova 1, Alexander A. Krasnikov 1, Tatyana D. Fershalova 1, Elena V. Baikova 1, Anastasia A. Petruk 1 & Yulia L. Yakimova 2
1 Central Siberian Botanical Garden SB RAS, Novosibirsk, Russia
2 State Research Center of Virology and Biotechnology VECTOR, Koltsovo, Russia
We studied the leaves of Begonia grandis Dryand. subsp. grandis, the northernmost and most cold-resistant representative of the predominantly tropical genus Begonia, by histochemical methods. In glandular and nonglandular trichomes as well as in the epidermal cells of B. grandis Dryand. subsp. grandis leaves, phenolic compounds, including flavonoids, as well as terpenoids and carbonyl compounds were detected. The patterns of phenolic compounds in the acetone and ethanol leaf exudates and in leaves as a whole were similar and contained oxalic, citric, and gallic acids, isoquercitrin, and orientin. Concentrations of phenolic compounds in the acetone and ethanol exudates constituted 0.10 % and 2.59 % of all phenolic compounds in the leaves, respectively. Antimicrobial effects of the aqueous ethanol extract and of the ethanol exudate against reference strains of Bacillus subtilis, Staphylococcus aureus, and Candida albicans were detected at the disc contents of 50.0 and 45.8 μg, respectively. The observed set of characteristics can be used in a targeted search for highly antimicrobial species of Begoniaceaе.
Карпова Е.А., Красников А.А., Фершалова Т.Д., Байкова Е.В., Петрук А.А., Якимова Ю.Л. Фенольные соединения и антимикробные свойства листьев Begonia grandis Dryand. subsp. grandis. С помощью гистохимических методов исследованы листья Begonia grandis Dryand. subsp. grandis – самого северного и холодоустойчивого представителя преимущественно тропического рода Begonia. В железистых и нежелезистых трихомах, а также в эпидермальных клетках листьев выявлены фенольные соединения, в том числе флавоноиды, а также терпеноиды и карбонильные соединения. Фенольные соединения в ацетоновом и этанольном экссудатах листьев, а также листьях в целом имеют сходный состав, содержащий щавелевую, лимонную и галловую кислоты, изокверцитрин и ориентин. Концентрации фенольных соединений в ацетоновом и этанольном экссудатах составляют 0,10 % и 2,59 % от суммы фенольных соединений листьев, соответственно. Выявлено антимикробное действие водно-спиртового экстракта и этанольного экссудата на тестовые штаммы Bacillus subtilis, Staphylococcus aureus и Candida albicans при содержании 50,0 мкг и 45,8 мкг на диск, соответственно. Изученная система признаков может быть использована для направленного поиска видов Begoniaceaе с высокой антимикробной активностью.
Keywords: Begonia grandis subsp. grandis, flavonoids, orientin, oxalic acid, antimicrobial activity, leaf exudate, trichomes, флавоноиды, ориентин, щавелевая кислота, антимикробная активность, экссудат листьев, трихомы
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