Botanica Pacifica. A journal of plant science and conservation Preprint
Article first published online: 13 JUL 2018 | DOI: 10.17581/bp.2018.07202
Diversity of fungal communities associated with mixotrophic pyroloids (Pyrola rotundifolia, P. media and Orthilia secunda) in their natural habitats
Ekaterina F. Malysheva1*, Vera F. Malysheva1 , Elena Yu. Voronina2 & Alexander E. Kovalenko1
1Komarov Botanical Institute of the Russian Academy of Sciences, Saint-Petersburg, Russia
2Lomonosov Moscow State University, Moscow, Russia
As it was shown, mixotrophic plants (MxP) strongly depend on their mycorrhizal fungi for carbon, at least at the early stages of life cycle, and have rather high specificity for mycobionts. However, the diversity of fungi associated with MxP and their role in plant’s life are still poorly known, especially under natural conditions. In the present study, the diversity of mycobionts of the three mixotrophic pyroloid species (Pyrola rotundifolia, P. media and Orthilia secunda) was investigated by sequencing nrITS from roots and rhizomes. At the same time, we studied ectomycorrhizal fungal communities of neighboring trees. The mycobiont diversity slightly differed berween the three species, but they also shared similar fungal taxa. The species of basidiomycete genera Tomentella, Piloderma, Russula and Mycena were dominant fungal partners of the studied pyroloids. The plants were also colonized by other ectomycorrhizal and saprotrophic basidiomycetes and ascomycetes. The research results showed MxP link to tree species by shared mycobionts and partial mycoheterotrophy by involvement into mycelial network. Thirty nine fungal taxa (at species and genera level) inhabiting pyroloid root system as mycobionts and root endophytes were detected. Their role for plant performance requires further investigation.
Малышева Е.Ф., Малышева В.Ф., Воронина Е.Ю., Коваленко А.Е. Разнообразие сообществ грибов, связанных с миксотрофными грушанковыми (Pyrola rotundifolia, P. media и Orthilia secunda) в их естественных местообитаниях. Миксотрофные растения (MxP) сильно зависят в углеродном питании от микоризных грибов, по крайней мере, на ранних стадиях развития, и обладают высокой специфичностью к микобионтам. Однако еще довольно мало известно о разнообразии грибов, связанных с MxP, и их роли в жизни растений, особенно в естественных экосистемах. В настоящей работе было исследовано разнообразие микобионтов трех видов грушанковых с миксотрофным типом питания (Pyrola rotundifolia, P. media и Orthilia secunda) путем выделения ДНК и последующего секвенирования nrITS из корней и корневищ. Одновременно изучался состав грибов, формирующих эктомикоризу с соседними деревьями. Обнаруженные сообщества микобионтов немного отличались у трех видов грушанковых, хотя были выявлены общие симбионты. Доминирующими партнерами в арбутоидной микоризе изученных видов растений явились базидиомицеты из родов Tomentella, Piloderma, Russula и Mycena. Растения были также колонизированы другими эктомикоризными и сапротрофными базидиомицетами и аскомицетами. Результаты исследования показали связь MxP с древесными растениями в природе благодаря наличию общих микобионтов, с помощью которых растения вовлекаются в единую мицелиальную сеть. Всего было выявлено 39 грибных таксонов (видового и родового рангов), обитающих в корневой системе грушанковых в качестве микобионтов и корневых эндофитов. Их роль в жизни растений требует дальнейшего изучения.
Keywords: Pyroleae, arbutoid mycorrhiza, mixotrophy, nrITS, operational taxonomic units, symbionts, endophytes, арбутоидная микориза, миксотрофия, операционные таксономические единицы, симбионты, эндофиты
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