Botanica Pacifica

Research paper

Botanica Pacifica. A journal of plant science and conservation Preprint
Article first published online: 26 NOV 2017 | DOI: 10.17581/bp.2017.06204

Plant regeneration from cotyledon and leaf explants of Ceropegia bulbosa Roxb., an endangered medicinal plant.

Balakrishnan Subbaiyan & Vellaichamy Thangapandian

PG and Research Department of Botany, Kongunadu Arts and Science College [Autonomous], Coimbatore, Tamil Nadu, India

An efficient protocol of callus induction, shoot multiplication and plant regeneration from cotyledon and leaf explants of Ceropegia bulbosa has been elaborated. The highest percentage (90.66) of callus induction was observed when cotyledonary explants cultured on MS medium having 0.5 mg/L 2,4-D in combination with 0.05 mg/L Kn followed by 86.25 % callus induction from leaf explant at 0.5 mg/l BAP in combination with 0.05 mg/l Kn. Shoot multiplication was observed, cotyledon derived callus cultured on 1.0 mg/L BAP in combination with 0.10 mg/L NAA could induce number of shoots (4.71) and their lengths (6.13 cm). Cotyledonary callus derived microshoots gave the highest rooting percentage (92.26 %) root numbers (13.14) and their lengths (8.87 cm) were observed on half-strength MS medium fortified with 0.4 mg/L IBA. In vitro derived plantlets were hardened in the greenhouse and successfully established into the soil.


Суббайан Б., Тангапандян В. Регенерация растений их семядолей и листьев Ceropegia bulbosa Roxb., редкого лекарственного растения. Разработан эффективный метод индукции каллюса, размножения побегов и регенерации растений из семядолей и эксплантатов листьев Ceropegia bulbosa. Наибольший процент (90,66) индукции каллюса наблюдался, когда семядольные эксплантаты культивировали на MS-среде, имеющей 0,5 мг/л 2,4-D, в комбинации с 0,05 мг/л Kn. 86,25 % индукции каллюса из эксплантата листьев отмечен при 0,5 мг/л BAP в сочетании с 0,05 мг/л Kn. Наблюдалось увеличение числа побегов: каллюс, полученный из семядолей, культивируемый на 1,0 мг/л BAP в сочетании с 0,10 мг/л NAA, мог индуцировать в среднем 4,71 побегов длиной 6,13 см. Побеги, полученные из каллюса семядоли на среде MS, обогащенной 0,4 мг/л IBA, дали наивысший процент корней (92,26 %) (общее число – 13,14) длиной в среднем 8,87 см. Полученные in vitro проростки прошли адаптацию в теплице и успешно внедрены в почву.

Keywords: Ceropegia, cotyledon, 2, 4-D, plant regeneration, microshoots, семядоля, регенерация растений, микропобеги

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