Botanica Pacifica. A journal of plant science and conservation Preprint
Article first published online: 08 JUL 2017 | DOI: 10.17581/bp.2017.06104
High frequency in vitro regeneration system for conservation of Barleria prionitis L., a threatened medicinal shrub
Vineet Soni1, Rakesh Kumari2 & P.L. Swarnkar3
1 Department of Botany, Mohanlal Sukhadia University, Udaipur-313001, Rajasthan, India
2 School of Life Science, Jaipur National University, Jaipur-302015, Rajasthan, India
3 Department of Botany, University of Rajasthan, Jaipur-302004, Rajasthan, India
A rapid and efficient plant propagation system through nodal and embryo cultures was developed for conservation of threatened shrub Barleria prionitis. Nodal explants exhibited high frequency shoot proliferation on Murashige and Skoog (MS) medium supplemented with 1.0 mg l-1 6-benzylaminopurine (BA) and 0.5 mg l-1 thidiazuron (TDZ). Microshoots were best rooted on half-strength MS fortified with 0.5 mg l-1 indole-3-butyric acid (IBA). Maximum conversion (63.6 %) of zygotic embryos into well rooted plantlets was achieved on half-strength MS supplemented with 20 mg l-1 sucrose, devoid of any growth regulator S. Plantlets with high PSII photochemical efficiency (Fv /Fm ≥ 0.8) were successfully shifted to natural conditions. The overall survival rate during acclimatization from in vitro growth to field transfer was 81 %. The developed micropropagation protocol can be successfully used for large-scale multiplication and conservation this high value medicinal plant species.
Сони В., Кумари Р., Шварнкар П.Л. Система интенсивной регенерации in vitro для сохранения Barleria prionitis L., редкого лекарственного кустарника. Разработана быстрая и эффективная система размножения растений через меристемные и эмбриональные культуры для сохранения угрожаемого кустарникового вида Barleria prionitis. Меристемные эксплантаты демонстрировали интенсивную пролиферацию побегов на среде Murashige и Skoog (MS), дополненную 1,0 мг/л 6-бензиламинопурином (BA) и 0,5 мг/л тидиазуроном (TDZ). Микропобеги лучше всего укоренялись на MS, обогащенной 0,5 мг/л индол-3-масляной кислотой (IBA). Максимальное преобразование (63,6 %) зиготных эмбрионов в укоренившиеся растения было достигнуто на MS, дополненной 20 мг/л сахарозы, лишенной какого-либо регулятора роста S. Растеньица с высокой фотохимической эффективностью PSII (Fv / Fm ≥ 0,8) были успешно перенесены в естественную среду. Общая выживаемость при акклиматизации от роста in vitro до переноса в естественную среду составляла 81 %. Разработанный протокол микроразмножения может быть успешно использован для крупномасштабного размножения и сохранения этих высокоценных лекарственных растений.
Keywords: micropropagation; embryo culture; in vitro rhizogenesis; chlorophyll fluorescence; acclimatization, микроразмножение, культура эмбрионов, корнеобразование in vitro, флуоресценция хлорофилла, акклиматизация
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