Malysheva E.F., Malysheva V.F., Kovalenko A.E., Pimenova E.A., Gromyko M.N., Bondarchuk S.N. & Voronina E.Y. Below-Ground Ectomycorrhizal Community Structure in the Postfire Successional Pinus koraiensis Forests in the Central Sikhote-Alin (the Russian Far East)

Research paper

Botanica Pacifica. A journal of plant science and conservation Preprint
Article first published online: 18 DEC 2015 | DOI: 10.17581/bp.2016.05102

Below-Ground Ectomycorrhizal Community Structure in the Postfire Successional Pinus koraiensis Forests in the Central Sikhote-Alin (the Russian Far East)

Ekaterina F. Malysheva ¹, Vera F. Malysheva ¹, Alexander E. Kovalenko ¹, Elena A. Pimenova ², Michael N. Gromyko ³, Svetlana N. Bondarchuk ³, Elena Yu. Voronina ⁴

¹ Komarov Botanical Institute of the Russian Academy of Sciences, Saint Petersburg, Russia
² Botanical Garden-Institute FEB RAS, Vladivostok, Russia
³ Sikhote-Alin State Nature Biosphere Reserve, Terney, Primorye Territory, Russia
⁴ Lomonosov Moscow State University, Moscow, Russia

This study focuses at the effect of forest fire on the extent of ectomycorrhizal (EcM) colonization of Pinus koraiensis. The EcM fungal diversity was estimated directly from mycorrhizas using molecular techniques. The revealed EcM fungal community was highly diverse, with 110 operational taxonomic units detected. The members of Russulaceae and Thelephoraceae were among the most dominant species in both the fire and control sites. The most diverse basidiomycete EcM genera at the burned sites were Tomentella, Russula, Piloderma, Inocybe, Suillus, Sebacina and Lactarius. Mycorrhizal colonization was found to be high for seedlings grown at the burned and the unburned sites. However, there was a rather significant shift between sites in terms of the taxonomic composition of EcM symbionts as well as the dominant fungal species. A vertical stratification of EcM fungal diversity at the control site and the sites of later succession stages was found to be more leveled. The results of the study indicate that EcM fungi apparently play an important role in the processes of regeneration of natural forest stands after fire at the studied area.

Малышева Е.Ф., Малышева В.Ф., Коваленко А.Е., Пименова Е.А., Громыко М.Н., Бондарчук С.Н., Воронина Е.Ю. Структура сообществ эктомикоризных грибов в почве кедровых лесов разных стадий послепожарной сукцессии в Центральном Сихотэ-Алине (российский Дальний Восток). Целью исследования было изучение эффекта воздействия пожара на степень эктомикоризной (ЭМ) колонизации корней Pinus koraiensis. Идентификация ЭМ компонента производилось непосредственно из микоризных окончаний с использованием молекулярных методов. Сообщества ЭМ грибов, ассоциированные с корнями P. koraiensis, были весьма разнообразны. Всего было обнаружено 110 операционных таксономических единиц (ОТE). Преобладающими по числу видов семействами во всех изученных сообществах, включая контроль, оказались Russulaceae и Thelephoraceae. Наиболее богатыми по видовой представленности родами были Tomentella, Russula, Piloderma, Inocybe, Suillus, Sebacina и Lactarius. Было установлено, что степень микоризной колонизации сеянцев в сообществах, подвергшихся пожару, и в не горевших участках была высокой. Выявлена разница между изученными сообществами в таксономическом составе и в составе доминирующих видов ЭМ симбионтов, образующих микоризную связь с сеянцами. Было оценено вертикальное распределение мицелия ЭМ грибов в горизонтах почвы. Показано, что это распределение выглядит более выровненным в ненарушенных лесах и на более поздних стадиях сукцессии. Результаты проведенного исследования показывают, что ЭМ грибы играют важную роль в процессах регенерации природных древостоев после пожаров.

Keywords: ectomycorrhiza, fungal diversity, nrITS, Korean pine, fire, Primorye Territory, эктомикориза, разнообразие грибов, кедр корейский, пожар, Приморский край

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